Dual Enzyme Multi-Layer Bioreactors: Analytical Modeling and Experimental Studies

Abstract

Enzymic reactors are developed for a variety of biomedical-biotechnological applications, including blood detoxification. For the latter, an appropriate approach is to use enzymes of the Mercapturic Acid Pathway. The first two enzymes of this pathway are Glutathione-S-Transferase (GST) and Y-Glutamyl Transpeptidase (YGT). Earlier, the performance of an immobilized GST reactor was investigated experimentally and theoretically. Here, the analytical model was extended to describe a dual-enzyme continuous packed-bed reactor (DCP), in which the two enzymes (Ei and E2) are arranged in alternating layers.