Abstract
Muscarinic agonists acting on bovine tracheal smooth muscle (BTSM) induce two separate cGMP signals, one at 20 sec associated with NO-sensitive-soluble-guanylyl-cyclase (NO-sGC) and another at 60 sec, linked to natriuretic-peptide-GC. The 20-sec-cGMP novel cascade starts with mAChRs, via unknown components, activates an NO-sGC. To unravel this cascade, in crude membranes isolated from intact BTSM strips exposed to muscarinic agonists, we detected GC activities increments at 20 sec and 60 sec. The 20-sec-GC is a NO-sensitive-GC, identified as α1β1-heterodimer. In reconstitution experiments with purified plasma membranes and cytosol, muscarinic agonists induced an NO-sGC migration in a dose-dependent manner, being inhibited by muscarinic antagonists displaying an M2AChR profile and blocked by PTX, suggesting the involvement of Go/Gi proteins. The NO-sGC related to migration was isolated and identified as an α1β1-heterodimer. This work shows that muscarinic agonists in BTSM induce a massive and selective α1β1-NO-sGC migration from cytoplasm to plasma membranes being responsible for the 20-sec-cGMP signal.
Acknowledgments
This work was supported by grants from the Consejo de Desarrollo Científico y Humanístico, Universidad Central de Venezuela (CDCH-UCV) PG-09-7401-2008/1 and PI 09.006464.2006/2 (to RGA), PI-09-6030-2007 (to ILB),and a grant from the Fondo Nacional de Ciencia y Tecnología (FONACIT) of Venezuela # S1-2002000411 (to ILB). The authors also thank Dr. Coral Wynter for the English version of this manuscript and Dr. Marcelo A. Alfonzo-González for theFigures style. During the submission of this manuscript, some reviewers’ comments were incorporated into themanuscript and we are grateful.
Declaration of interest
This work was supported by grants from the Consejo de Desarrollo Científico y Humanístico, Universidad Central de Venezuela (CDCH-UCV) PG-09-7401-2008/1 and PI 09.006464.2006/2 (to RGA), PI-09-6030-2007 (to ILB),and a grant from the Fondo Nacional de Ciencia y Tecnología (FONACIT) of Venezuela # S1-2002000411 (to ILB). The authors also thank Dr. Coral Wynter for the English version of this manuscript and Dr. Marcelo A. Alfonzo-González for theFigures style. During the submission of this manuscript, some reviewers’ comments were incorporated into themanuscript and we are grateful.