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Journal of Receptor Research Volume 3, 1983 - Issue 1-2
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Research Article

Synthesis, Purification by HPLC and Characterization of Monoiodoglucagon Labeled with Carrier Free 125I: Use as Glucagon Receptor Probe

Lutz Birnbaumer Department of Cell Biology, Baylor College of Medicine, Houston, Texas, 77030, USA
,
Theodore L. Swartz Department of Cell Biology, Baylor College of Medicine, Houston, Texas, 77030, USA
,
Francisco J. Rojas Department of Cell Biology, Baylor College of Medicine, Houston, Texas, 77030, USA
&
Alan J. Garber Department of Cell Biology, Baylor College of Medicine, Houston, Texas, 77030, USA
Pages 3-20 | Published online: 26 Sep 2008
 
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Abstract

Methods are described: a to iodinate glucagon with carrier-free 125I using Iodogen© as the oxidizing agent and, b to purify from the reaction medium a mixture of [125I-Tyr10] monoiodoglucagon and [125I-Tyr13] monoiodoglucagon using reverse phase high pressure liquid chromatography (HPLC).

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