Development of a Novel TNFα Ligand-Receptor Binding Assay for Screening Natchem^TM Libraries

Abstract

The TNFα receptor is a major therapeutic target since over production of TNFα plays a key role in the development of septic shock following bacterial infection and has been implicated in the pathogenesis of many inflammatory disorders such as rheumatoid arthritis. To screen our NATCHEM^TM Libraries for novel natural product inhibitors of this target we have developed a sensitive immobilised TNFα receptor binding assay based on a commercially available recombinant soluble TNFα receptor (p55) and [¹²⁵I]-TNFα.