Urinary lipid and protein oxidation products upon halothane, isoflurane, or sevoflurane anesthesia in humans: potential biomarkers for a subclinical nephrotoxicity

Abstract

Objective: To investigate whether lipid and protein oxidation products are elevated and correlated with routine clinical markers of hepatic and renal function in patients anesthetized with halothane, isoflurane, or sevoflurane.

Methods: Urine and blood samples were collected from patient groups. Excretion of aldehydes, acetone, and o,o’-dityrosine was measured before and after anesthesia. Blood samples were analysed for clinical markers.

Results: Urinary concentrations of aldehydes, acetone, o,o’-dityrosine and glucose were significantly increased after anesthesia in halothane and sevoflurane groups earlier than clinical markers. Significant correlations were found in sevoflurane group.

Conclusion: Lipid and protein oxidation contributes to subclinical sevoflurane nephrotoxicity. Oxidation products may serve as early biomarkers.

Keywords::

• Aldehydes
• acetone
• dityrosine
• tubular damage
• clinical markers

Acknowledgements

The Biochemistry Laboratory of Hacettepe University Hospitals is acknowledged for measuring clinical parameters except urinary protein and glucose.

Declaration of interest

Hilmi Orhan was supported with a grant of the Scientific and Technical Research Council of Turkey (TUBITAK-NATO A2) during the course of the study. The authors report no declaration of interests.