Longitudinal study of [D₁₀]phenanthrene metabolism by the diol epoxide pathway in smokers

Abstract

The extent of metabolism of [D₁₀]phenanthrene to [D₁₀]r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4-tetradeuterophenanthrene ([D₁₀]PheT) could be a biomarker of human metabolic activation of carcinogenic polycyclic aromatic hydrocarbons, leading to identification of smokers particularly susceptible to lung cancer. The longitudinal stability of [D₁₀]PheT was evaluated in 24 cigaret smokers given 7–8 oral doses of [D₁₀]phenanthrene (10 µg) over 5.5 months. [D₁₀]PheT in 6 h urine was quantified after each dose. The overall coefficient of variation for 24 subjects was (mean ± S.D.) 27.4% ± 8.83%. Thus, a single administration of [D₁₀]phenanthrene is likely sufficient to determine a smoker’s ability to metabolize it to [D₁₀]PheT.

• Carcinogenic polycyclic aromatic hydrocarbons
• cigaret smokers
• [D₁₀]phenanthrene
• [D₁₀]r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4-tetradeuterophenanthrene ([D₁₀]PheT)
• urinary metabolites