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Abstract
Objective: We performed comprehensive proteomic analyses of articular cartilage by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and searched for candidate biomarkers for osteoarthritis (OA).
Methods: Articular cartilage was collected from patients with OA or femoral neck fracture for the control group. Molecular variations were detected by the iTRAQ method, and quantitative analyses were performed by western blot.
Results: Using the iTRAQ method, we identified 76 proteins with different expression levels in OA patients and the control group. Among these proteins, we selected LECT2 (leukocyte cell-derived chemotaxin-2), BAALC (brain and acute leukemia, cytoplasmic), and PRDX6 (peroxiredoxin-6), which had not been reported as biomarkers for OA.
Conclusions: Use of these proteins in combination with conventional OA biomarkers may better reflect the grade and prognosis of OA.
Acknowledgements
We thank everyone in the orthopaedic surgery department at Fujita Health University Hospital for provision of cartilage. We thank Mrs. Sato for excellent technical assistance.
Contributions
All authors were involved in drafting the article or revising it critically for important intellectual content, and all authors approved the final version for publication.
Dr Yamada had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.
Study design. Ikeda, Ageta.
Acquisition of data. Ikeda, Ageta
Analysis and interpretation of data. Ikeda, Ageta.
Manuscript preparation. Ikeda, Ageta, Tsuchida, Yamada
Statistical analysis. Ikeda, Ageta.