Identification of potential biomarkers for post-traumatic complications released after trauma–hemorrhage from murine Kupffer cells and its investigation in lung and liver

Abstract

Context: Early diagnosis of complications after severe trauma by specific biomarkers remains difficult.

Objective: Identify potential new biomarkers for early diagnosis of post-traumatic complications.

Material and methods: Mice underwent pressure-controlled hemorrhage or sham procedure. Four hours later, genome-wide expression of isolated Kupffer cells was compared with controls using Affymetrix-Genechip-Expression-Analysis and real-time-PCR.

Results: Expression analysis and real-time-PCR revealed a significant increase of gene expression of Cxcl10, Il4ra, Csf2rb2, Lcn2, and Gbp5.

Conclusion: Cxcl10, Il4ra, Csf2rb2, Lcn2, and Gbp5 might represent new biomarkers for early diagnosis of post-traumatic complications, if they are linked to the development of post-traumatic complications.

Keywords:

• Csf2rb2
• Cxcl10
• Gbp5
• Il4ra
• Lcn2
• multiple trauma

Acknowledgements

The authors thank Ms. Claudia Pütz for her excellent technical assistance. Affymetrix whole transcript expression and statistical analysis used or referred to in this publication were generated by MFT Services of the University of Tuebingen, Germany.

Disclosure statement

The authors report that they have no conflicts of interest.