Allelic loss at PTEN locus leads to progression of colorectal carcinoma among North Indian patients

Abstract

We evaluated the loss of heterozygosity (LOH) at 10q^23.3 locus of microsatellite markers; D10S198, D10S192, and D10S541 of PTEN gene in 223 North Indian colorectal cancer (CRC) specimens. DNA was isolated and microsatellite-specific markers polymerase chain reaction was performed. Out of total 223 cases 102 showed LOH for at least one of the locus. In addition, thereto a significant association was found with the clinicopathologic features like grade of differentiation, clinical stage, invasion, lymph node invasion, and the clinical outcome (p < 0.05). These data argue that the given markers to check the possible LOH of PTEN gene at locus 10q^23.3 could be considered as one of the diagnostic markers in CRC.

Keywords:

• Colorectal cancer
• loss of heterozygosity
• microsatellite marker
• PCR
• PTEN

Disclosure statement

The authors have no conflict of interest.

Funding information

The authors acknowledge the Department of Biotechnology (DBT), Govt. of India for providing the financial assistance for this study.