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Abstract
Phosphorylase a activity in the isolated perfused rat heart was about 20 % of the total enzymes. Various standard freezing and thawing procedures or seasonal variation did not alter the basal level of the enzyme activity and is not considered to be the effect of anoxia. Blood and noradrenaline were found to increase phosphorylase activity in the heart. Perfusing the heart with low concentrations of glucose increased phosphorylase activity without changes in cardiac function. A linear relationship between noradrenaline-induced changes in phosphorylase activity and contractile force in the rat heart was observed.