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Abstract
(1) The metabolism of the glucosamine was studied in the liver of S. canicula, after injection of D-(1-14C) glucosamine into the animal.
(2) The labelled acid-soluble derivatives were separated by ion exchange columns and characterized by chromatography and electro-phoresis, and were identified as glucosamine, glucosamine 6-P, N-acetyl-glucosamine, N-acetylmannosamine, N-acetylneuraminic acid, N-acetyl-glucosamine 6-P, N-acetylglucosamine 1-P, N-acetylmannosamine 6-P, UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine.
(3) The variation with time after glucosamine injection of the radioactivity of the fractions separated on Dowex 1-X4 was investigated. This study showed a decrease of the radioactivity in the fraction 1 (glucosamine and glucosamine 6-P), an increase in the fraction II (N-acetylneuraminic acid) and the fraction IV (UDP-N-acetylhexosamines), and a stability in the fraction III (phosphorylated-N-acetylhexosamines).
(4) The absence of label in neutral hexoses and their phosphorylated derivatives was interpretated as due to the weak activity of the glucosamine 6-P isomerase, which is positively modulated by the N-acetyl-glucosamine 6-P.