Kinetics of Mg²⁺-ATPase inhibition by benthiocarb and its recovery by oximes and L-cysteine in brain of albino rat (Wistar strain)

Abstract

Effects in vitro of benthiocarb on rat brain Mg²⁺-ATPase were studied to elucidate the interaction of benthiocarb with Mg²⁺-ATPase. Based on IC₅₀ values, it was evident that Mg²⁺-ATPase was sensitive to benthiocarb. Non-competitive inhibition with respect to activation by ATP was indicated by decreased maximal velocity (V) without change in Michaelis Menten constant (K_m). It was also noted that pyridine-2-aldoxime (30 μM), pyridine-4-aldoxime (35 μM) and L-cysteine (90 μM) neutralized the inhibition of benthiocarb (8 μM).