The Na⁺/H⁺ exchanger role in the intracellular pH regulation of human platelets

Abstract

The intracellular pH of human platelets was measured with a fluorescent intracellular probe. When platelets were in basal conditions (pHo 7.4, [Na⁺]o 140 mm) the pHi was 6.98 ± 0.04. Five minutes after the addition of EIPA 60 μm, an inhibitor of Na⁺/H⁺ exchange, the pHi fell in 0.075 ± 0.022 pH units (P < 0.05).

Preincubation in a sodium free, acid medium (pHo 6.3) induced a cell acidification to pH 6.61 ± 0.03 (P < 0.01). Preacidified platelets showed a recovery in sodium-containing solution that is a function of [Na⁺]o.

The initial rate of recovery depends on [Na⁺]o in a Michaelis-Menten fashion, showing a Km of 35.6 mm and a Vmax of 0.213 pH units/min.

These results show that the pHi is maintained in human platelets by a Na⁺/H⁺exchange that is active even in basal conditions. The properties of the Na⁺/H⁺ exchanger in human platelets and in the less accessible smooth vascular cells are similar; generalized pathological alterations, like hypertension, could be reflected by both tissues.