Antitumor activity of polysaccharides isolated from Patrinia heterophylla

Abstract

The research investigated the effect of Patrinia heterophylla Bunge (Valerianaceae) polysaccharides (PHB-P1) on U14-bearing mice. The tumor weight of mice treated with PHB-P1 (30, 60 mg/kg body weight) was significantly lower than that of the control group, a decrease of serum lactate dehydrogenase (LDH) activity was observed, and the serum alkaline phosphatase (AKP) level was increased slightly. The number of apoptotic tumor cells was significantly increased in the mice by treatment of PHB-P1 (30, 60 mg/kgbw). Cell cycle analysis showed the accumulation of tumor cells in the G2/M phase and a relative decrease of the S phase. By the immunohistochemical analysis, PHB-P1 (30, 60 mg/kgbw) might up-regulate the expression of p53 and Bax, and significantly inhibited the expression of Bcl-2 in tumor tissues. In conclusion, PHB-P1 could inhibit tumor growth and induce tumor cell apoptosis.

Keywords::

• Antitumor
• apoptosis
• Patrinia heterophylla Bunge
• polysaccharides
• U14

Acknowledgements

The authors would like to thank Zhao Jian-Cheng who authenticated the Patrinia heterophylla samples used. Geng Guo-Xia and Lu Wen-Zong equally contributed to the work.

Declaration of interest

This study was supported by the Science and Technology Development Fund of Science and Technology Investing Company, Qinhuangdao, China (D08).