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Research Article

In vivo wound-healing efficacy and antioxidant activity of Achyranthes aspera in experimental burns

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Pages 892-899 | Received 16 Aug 2011, Accepted 11 Nov 2011, Published online: 06 Apr 2012
 

Abstract

Context: The floral richness of the North-East Indian region cannot be neglected in context to its medicinal importance. Achyranthes aspera Linn. (Amaranthaceae; Prickly Chaff flower) is an indigenous plant species of this region. Although the local traditional healers have ethnomedical knowledge on the use of this plant, there is no scientific study on wound-healing activity of this plant.

Objective: The healing efficacy of methanol leaf extract of A. aspera (MEAA) in granulation tissue of burn wound and its antioxidant activity are investigated.

Materials and methods: Methanol extract of leaves of A. aspera was used for compounding 5% (w/w) ointment, which was applied topically twice daily in experimental burn wound in rats. Healing potential was assessed by rate of wound contraction, antioxidant and biochemical assay which was supported by gelatin zymography and histopathology.

Results: In the present study, 5% ointment of A. aspera showed significant (p < 0.05) wound healing, which was evident by wound contraction, elevation of various antioxidant enzymes viz. SOD, catalase, vitamin C and prohealing and biochemical parameters like hydroxyproline and protein content than the control animals. Up-regulated expression of matrix metalloproteinases (MMP-2 and 9) was also observed by gelatin zymography. Histopathological examination of the granulation tissues in the A. aspera-treated animals showed collagen deposition, fibroblast proliferation and formation of epidermis.

Discussion and conclusion: The methanol leaf extract of A. aspera showed excellent wound-healing activities which has great potential for development of plant-based product.

Acknowledgments

The authors are grateful to Defence Research Development Organization (DRDO), Govt. of India, New Delhi for financial help, the Director of Research (Vety) and CIF, FVSc, Khanapara for providing necessary facilities. Thanks are also due to the Division of Pharmacology, DRDE, Gwalior, for conducting toxicity study.

Declaration of interest

The authors report no declaration of interest. The authors alone are responsible for the content and writing of the paper.

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