Abstract
Two ecdysteroids, 5β-ecdysone And 5P-hydroxyecdysterone, were separated from hypogeous organs of five populations of Helleborus odorus ssp. laxus (Host) Merxm. & Podl. (Ranunculaceae). The ecdysteroid content is higher during the vegetative growth (summer) AND lower during flowering (spring) AND quiescency (autumn-winter). Plants growing in short grass plains have an ecdysteroid content higher (30-40%) than plants from oak AND chestnut woods.