Inhibition of carbonic anhydrase isozymes I and II with natural products extracted from plants, mushrooms and honey

Abstract

Different natural products and secondary metabolites from mushrooms, teas, honeys, mosses, plants and seaweeds were investigated for their in vitro inhibitory effects on human carbonic anhydrase (hCA, E.C.4.2.1.1) isoforms I and II. Inhibition data were correlated with the total phenol content in the extract and investigated with the pure compounds believed to be responsible for this activity. Methanolic extracts were prepared for 17 such pure chemicals present in the natural products and for 41 diverse natural products. The IC₅₀ values were in the range of 0.11–66.50 μg/mL against hCA I and of 0.09–54.54 μg/mL against hCA II, respectively. The total phenol content was in the range of 0.02–1318.96 (as milligrams of gallic acid equivalents) per gram of sample. These data offer new insights on possible novel classes of CA inhibitors based on natural products, possessing a range of chemical structures not present in the classical inhibitors with pharmacological applications, such as the sulfonamides and sulfamates.

Keywords::

• Carbonic anhydrase
• natural products
• phenol
• inhibition
• isoform I and II

Acknowledgments

We also thank Assoc. Prof. Dr. Turan ÖZDEMIR for providing and identifying the moss sample Biology Department, Karadeniz Technical University, and also thanks to Assoc. Prof. Dr. Ömer ERTÜRK for providing and identifying the seaweeds sample Biology Department, Ordu University. Work from Supuran lab was financed by two FP7 EU projects (Metoxia and Gums and Joints).

Declaration of interest

This study was supported by Research Fund of Karadeniz Technical University (Project No: 2009.111.002.5). One of the authors (H. Sahin) would like to thank TUBİTAK, BİDEB, TURKEY, for the financial support given to him.