Enolase and the Arsonomethyl Analogue of 2-Phosphoglycerate

Abstract

(RS)-3-Arsono-2-(hydroxymethyl)propionic acid was synthesized by the action of alkaline arsenite on 3-bromo-2-(bromomethyl)propionic acid. It is a substrate for yeast enolase (EC 4.2.1.11) with a K_m of 6.5 mM (for 2-phospho-D-glycerate K_m = 0.08 mM). The catalytic constant of the enzyme with the arsonomethyl analogue is 230 times lower than with 2-phosphoglycerate.

Key Words: :

• Enolase
• 2-phosphoglycerate
• arsonomethyl analogue