Reversible Inhibition of Enzymatic Systems Involving Covalent Intermediates

Abstract

Reversible inhibition phenomena are analyzed for enzymatic systems involving covalent intermediates, where the inhibitor can bind to the pure enzyme, the Henri-Michaelis complex or the covalent intermediate, or to two or three of these enzyme forms. Classical competitive, non-competitive or un-competitive phenomena can be observed in some cases but unexpected features are also observed. Complex phenomena sometimes prevail where increased k_cat/K_m values can be accompanied by decreased or increased k_cat values.

Key Words: :

• Mechanism
• reversible inhibition.