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Original Article

Dual Inhibitory Effects of Dimethyl Sulfoxide on Poly(ADP-Ribose) Synthetase

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Pages 239-250 | Received 03 Apr 1998, Accepted 23 Jul 1998, Published online: 02 Jul 2009
 

Abstract

Dimethyl sulfoxide (DMSO), a solvent popularly used for dissolving water-insoluble compounds, is a weak inhibitor of poly(ADP-ribose) synthetase, that is a nuclear enzyme producing (ADP-ribose)n from NAD+. The inhibitory mode and potency depend on the concentration of substrate, NAD+, as well as the temperature of the reaction; at micromolar concentrations of NAD+, the inhibition by DMSO is biphasic at 37°C, but is monophasic and apparently competitive with NAD+ at 25°C. DMSO, on the other hand, diminishes dose-dependently and markedly the inhibitory potency of benzamide and other inhibitors. Other organic solvents, ethanol and methanol, also show a biphasic effect on the synthetase activity at different concentrations.

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