High quality genomic DNA extraction from postmortem fetal tissue

Abstract

Objective: We examined the yield and quality of genomic deoxyribonucleic acid (DNA) extracted from various postmortem fetal tissues. Methods: Fetal tissues were collected at the time of autopsy, and DNA was subsequently extracted. The yield and DNA quality was assessed using ultraviolet spectrometry and agarose gel electrophoresis. We used polymerase chain reaction (PCR) to assess the DNA extracted for genomic testing. Results: The median (range) gestation of the fetuses was 22 (16–41) weeks and the postmortem interval was 5.5 (2–10) days. Non-degraded genomic DNA was successfully extracted from all fetal tissues. Liver tissue had the lowest quality and muscle the highest quality. DNA yield or purity was not influenced by the postmortem interval. Conclusion: High quality genomic DNA can be extracted from fetal muscle, despite postmortem intervals of several days.

Keywords::

• DNA
• genetic testing
• postmortem
• stillbirth

Declaration of Interest: ST and AMT conceived the idea, developed grant application and study protocol along with NJS, DA, CM and PBM. SA performed DNA extractions under the supervision of CM and PBM and wrote the first draft of the paper. All authors contributed to the manuscript and approved the final version for submission. The study is funded by Well being of Women’s (WoW) and Stillbirth and Neonatal Deaths (SANDS) charity. ST is supported by a clinician scientist award from the National Institute for Health Research. For PBM this work forms part of the research themes contributing to the translational research portfolio for Barts and the London Cardiovascular Biomedical Research Unit, which is supported and funded by the National Institute for Health Research.