Syngeneic transplantation of newborn splenocytes in a murine model of neonatal ischemia-reperfusion brain injury

Abstract

Objective: Neonatal hypoxic-ischemic encephalopathy (HIE) is caused by brain injury that occurs in a developing fetus or infant. Stem cell transplantation can reportedly induce functional recovery in animal models of HIE. Murine neonatal splenocytes are enriched with immature blood stem cells and are used for the investigation of murine models of syngeneic transplantation. The aim of this study was to investigate the therapeutic potential of newborn splenocytes in a murine model of neonatal ischemia-reperfusion brain injury.

Methods: C57BL/6N mice (postnatal day 7) underwent right common carotid artery occlusion with an aneurysm clip. Following hypoxic exposure, reperfusion was achieved by unclamping the artery. Newborn splenocytes were transplanted intravenously at 3 weeks after injury.

Results: The splenocytes transplanted group tended to show an improvement in behavioral tests, but it was not significantly different compared with the control groups. The transplanted cells were localized in various organs including injured brain tissue over 3 weeks. In the penumbra region of the brain, vascular endothelial growth factor (VEGF) expression was upregulated after transplantation.

Conclusions: These results showed that syngeneic transplantation of newborn splenocytes achieved the long-term survival of the grafts and exerted influence the microenvironment in the injured brains of mice.

• Cerebral palsy
• stem cells
• umbilical cord blood

Acknowledgements

The authors thank Ms Kimiko Takaishi, Mr Yasushi Okada and Dr Atsuya Nobumoto for their assistance.

Declaration of interest

This work was partly supported by a Grant-in-Aid for Young Scientists (B), Ministry of Education, Culture, Sports, Science, and Technology, Japan.