Effect of time-varied magnetic field on inflammatory response in macrophage cell line RAW 264.7

Abstract

The aim of this feasibility study was to determine the effectiveness of a commercially manufactured magnetic field (MF) device as an adjunct to pharmaceuticals during acute phase inflammatory response. The goal was to determine if inflammatory response interleukins IL-1, IL-6, IL-10, and tumor necrosis factor-alpha (TNF-α) would be affected by a 30 Hz time-varying magnetic field (MF). RAW 264.7 macrophage-like cells were induced with Gram-negative bacteria lipopolysaccharide (LPS) to initiate an acute inflammatory reaction. Following lipopolysaccharide (LPS) treatment, both inflamed and control cells were exposed to MF for 1 h. After MF exposure, cytokines of interest were measured and compared with controls. Outcomes revealed that LPS challenged cells continuously exposed to a 30 Hz time-varying magnetic field for 1 h demonstrated significant changes compared with controls. From cytokine test it was determined that MF exposure significantly decreased levels of IL-6 and IL-10 compared to unexposed counterparts. TNF-α production was significantly affected when MF was applied to cells only, but not to inflamed cells. Results suggest that the biological effect of 1 h exposure to a 30 Hz time-varied magnetic field may act to down regulate specific cytokines in an inflamed environment.

Keywords::

• Cytokine production
• Lipopolysaccharide (LPS) induction
• IL-1
• IL-6
• IL-10
• Tumor necrosis factor alpha

Acknowledgement

This work was sponsored by the Caryl Guth Energy Medicine Fund.

Declaration of interest The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.