Effects of pH on the Zeta-Potentials of Bone in Vitro

W. K. Elwood Department of Anatomy, College of Medicine, University of Kentucky, Lexington, KY, 40536-0084; Department of Oral Biology, College of Medicine, University of Kentucky, Lexington, KY, 40536-0084; Department of Anatomy and Wenner-Gren Research Laboratory, College of Medicine, University of Kentucky, Lexington, KY, 40536-0084; Department of Statistics College of Medicine, University of Kentucky, Lexington, KY, 40536-0084

S. D. Smith Department of Anatomy, College of Medicine, University of Kentucky, Lexington, KY, 40536-0084; Department of Oral Biology, College of Medicine, University of Kentucky, Lexington, KY, 40536-0084; Department of Anatomy and Wenner-Gren Research Laboratory, College of Medicine, University of Kentucky, Lexington, KY, 40536-0084; Department of Statistics College of Medicine, University of Kentucky, Lexington, KY, 40536-0084

H. E. McKean Department of Anatomy, College of Medicine, University of Kentucky, Lexington, KY, 40536-0084; Department of Oral Biology, College of Medicine, University of Kentucky, Lexington, KY, 40536-0084; Department of Anatomy and Wenner-Gren Research Laboratory, College of Medicine, University of Kentucky, Lexington, KY, 40536-0084; Department of Statistics College of Medicine, University of Kentucky, Lexington, KY, 40536-0084

Abstract

Zeta-potentials of bone in vitro were determined at pH 3, 4, 5, 6, 7, 8, 9, and 10 or in the reverse order with two different buffer series before and after deep-freeze storage (−80° C) in 0.01 M HEPES (pH 7.4) containing 5.2% (W/V) glucose. The buffer series used, the pH sequence, prestorage subjection to a pH sequence, and storage itself all proved to be important influences in determining the ξ-potential characteristics of endosteal bone surface even though no isoelectric point was found

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Effects of pH on the Zeta-Potentials of Bone in Vitro

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Effects of pH on the Zeta-Potentials of Bone in Vitro

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