Confirmation of Emit Cannabinoid Assay Results by Bonded Phase Adsorption with Thin Layer Chromatography

Nicholas J. Willson Dept. of Neuropathology and Neurotoxicology, New York State Psychiatric Institute and Div. of Neuropathology College of Physicians and Surgeons of Columbia University, New York, New York, 10032

Michael J. Kdgan Dept. of Neuropathology and Neurotoxicology, New York State Psychiatric Institute and Div. of Neuropathology College of Physicians and Surgeons of Columbia University, New York, New York, 10032

David J. Pierson Dept. of Neuropathology and Neurotoxicology, New York State Psychiatric Institute and Div. of Neuropathology College of Physicians and Surgeons of Columbia University, New York, New York, 10032

Eric Newman Dept. of Neuropathology and Neurotoxicology, New York State Psychiatric Institute and Div. of Neuropathology College of Physicians and Surgeons of Columbia University, New York, New York, 10032

Abstract

Clinical urine specimens were screened for the presence of cannabinoids using the EMIT Cannabinoid Assay. Aliquots of these samples were also analyzed for 11-nor- Δ9-tetrahydrocannabinol-9-carboxylic acid (THCA), the major cannabis metabolite in urine, by a technique which combines bonded phase adsorption (BPA) and thin layer chromatography (TLC). A 100% agreement between EMIT and BPA-TLC results was observed when at least 20 mL of urine was assayed by BPA-TLC. Bonded phase adsorption coupled with thin layer chromatography appears to be a suitable technique for the confirmation of positive EMIT Cannabinoid Assay results.

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Confirmation of Emit Cannabinoid Assay Results by Bonded Phase Adsorption with Thin Layer Chromatography

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Confirmation of Emit Cannabinoid Assay Results by Bonded Phase Adsorption with Thin Layer Chromatography

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