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Abstract
Dermal absorption studies with living animals have several disadvantages. The present study reports the use of our automated in vitro dermal absorption (AIDA) procedure developed inhouse as a potential alternative to in vivo testing. Finite-dose AIDA studies were conducted with the pesticides DEET, 2,4-D, Diazinon, and DDT, these compounds being chosen for their wide range of lipo-philicities. Absorption (percentage recovery in receiver solution) in the human-derived tissue-cultured skin, Testskin, was similar to that in pig skin for three of the four test compounds. Testskin was 2.5 times more permeable than pig skin to 2,4-D. Continuous-dose AIDA studies conducted with the swimming pool stabilizer, cyanuric acid (CYA), demonstrated minimal CYA absorption through rat, hairless guinea pig, human, and Testskin. Total cumulative absorption of CYA by 24 hr in Testskin and human skin was 0.02 µg CYA/cm2 in both cases.