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Original Article

Determination of aflatoxin B1 levels in Iranian rice by ELISA method

, , , &
Pages 125-128 | Received 24 May 2015, Accepted 17 Jul 2015, Published online: 23 Sep 2015
 

Abstract

This study was carried out to detect the presence of aflatoxin B1 (AFB1) in 40 samples of Tarom rice from Iran. Enzyme-linked immunosorbent assay (ELISA) was applied to analyze AFB1 in the samples. All the analyses were conducted twice. Aflatoxin B1 was found in all rice samples, the concentration of AFB1 ranged from 0.29 to 2.92 µg/kg. The AFB1 concentration mean in the rice samples produced in 2013 was higher (P < 0.05) than the findings in rice in 2012.. However, 25 of the 40 samples exceeded the maximum prescribed limit, i.e. 2 µg/kg of European Union Regulations and also none of the samples reached the maximum prescribed limit 5 µg/kg of the Institute of Standards and Industrial Research of Iran (ISIRI) for aflatoxin B1. Although, rice is ranked the second among cereal staples consumed food in Iran and many countries, it can make a serious health problem for people even for a small amount of aflatoxin.

Declaration of interest

The authors declare that there are no conflicts of interest.

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