Proteomic profiling and protein identification by 2-DE-MS in the liver of Carassius auratus exposed to Yongcheng coalmine subsidence area

Abstract

Proteomic profile of the Carassius auratus liver was constructed to evaluate contamination degree of aquatic organism exposed to coalmine subsidence area. The results revealed that 55 proteins were up-regulated and 51 down-regulated among 160 differentially expressed proteins. These proteins were mainly involved in the physiological activities, such as granzyme B signaling, dermatan sulfate biosynthesis and cytotoxic T lymphocyte mediated apoptosis of target cells. Meanwhile, they were related to two network interaction modules. One was cell adhesion and migration, angiogenesis, DNA repair, and the other was small molecule metabolism, structural molecule activity and defense response. Ingenuity Pathway Analysis (IPA) was further utilized to predict differential proteins molecular activation, including tnf, tp53, Anxa3, fgf2 and mvp. The result sheds light on liver protein expression profiles of C. auratus, and provides important data for controlling and managing water pollution from heavy metals.

• Carassius auratus
• heavy metals
• liver
• mass spectrometry
• two-dimensional gel electrophoresis