Abstract
To assess effects of carbon nanoparticle (CNP) exposure on renal epithelial cells, fullerenes (C60), single-walled carbon nanotubes (SWNT), and multi-walled carbon nanotubes (MWNT) were incubated with a confluent renal epithelial line for 48 h. At low concentrations, CNP-treated cells exhibited significant decreases in transepithelial electrical resistance (TEER) but no changes in hormone-stimulated ion transport or CNP-induced toxicity or stress responses as measured by lactate dehydrogenase or cytokine release. The changes in TEER, manifested as an inverse relationship with CNP concentration, were mirrored by an inverse correlation between dose and changes in protein expression. Lower, more physiologically relevant, concentrations of CNP have the most profound effects on barrier cell function and protein expression. These results indicate an impact of CNPs on renal epithelial cells at concentrations lower than have been previously studied and suggest caution with regard to increasing CNP levels entering the food chain due to increasing environmental pollution.
Declaration of interest: These studies were supported by a grant from NIGMS (R01GM085218), NIEHS (RC2ES018810), and the IUPUI Undergraduate Research Opportunities Program. The authors gratefully acknowledge the technical support of Heather Ringham and Susana Ado Ntim, and sincerely thank Dr Alain Vandewalle (INSERM U478, Paris, France) for providing the mpkCCDcl4 cell line. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.