Mechanisms of genotoxicity. A review of in vitro and in vivo studies with engineered nanoparticles

Abstract

Engineered nanoparticles (NPs) are widely used in different technologies but their unique properties might also cause adverse health effects. In reviewing recent in vitro and in vivo genotoxicity studies we discuss potential mechanisms of genotoxicity induced by NPs. Various factors that may influence genotoxic response, including physico-chemical properties and experimental conditions, are highlighted. From 4346 articles on NP toxicity, 112 describe genotoxicity studies (94 in vitro, 22 in vivo). The most used assays are the comet assay (58 in vitro, 9 in vivo), the micronucleus assay (31 in vitro, 14 in vivo), the chromosome aberrations test (10 in vitro, 1 in vivo) and the bacterial reverse mutation assay (13 studies). We describe advantages and potential problems with different methods and suggest the need for appropriate methodologies to be used for investigation of genotoxic effects of NPs, in vitro and in vivo.

Keywords::

• nanoparticle toxicity
• DNA damage
• DNA oxidation
• mechanisms of genotoxicity
• primary genotoxicity
• secondary genotoxicity
• nanoparticle properties
• nanotoxicology

Acknowledgments

Supported by EC FP7 [Health-2007-1.3-4], Contract no: 201335, www.nanotest-fp7.eu.