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Abstract
The complete mitochondrial genome sequence of the Chinese catfish Hemibagrus macropterus was determined using long polymerase chain reaction (PCR). The complete mtDNA sequence was 16,530 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region (D-loop), the gene composition and order of which were identical to most of the other vertebrates. Two conserved sequence blocks (CSB-2 and CSB-3) in the control region can be easily identified in all available bagrid mitogenomes. The overall base composition of the heavy strand was 31.6% A, 27.7% T, 25.8% C, and 14.8% G, with an AT content of 59.3%. The mitogenome sequence of H. macropterus could be contributed to elucidate the evolutionary relationships among the bagrid catfishes, the systematics and evolution of which group remain poorly explored to date.
Acknowledgements
The authors thank Mr Jun Ma for the laboratory work.
This work was supported by the grants from the National Natural Science Foundation of China (31071903) and the Fundamental Research Funds for the Central Universities (XDJK2011B010).
Declarations of interest: The authors declare no conflict of interest. The authors alone are responsible for the content and writing of the paper.
