Abstract
The blue-spotted stingray Neotrygon kuhlii is a small stingray distributed in the Indian and West Pacific Ocean. It was considered a species complex by morphological and molecular evidences. In this study, we firstly presented the complete mitochondrial genome of N. kuhlii. It is 18,039 bp in length, including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and 1 control region, with the typical gene order in the mitogenomes of vertebrates. The total base composition is 31.9% A, 26.4% C, 13.1% G and 28.6% T, with an A + T bias of 60.5%. Alignment results demonstrated that the mitochondrial sequences of N. kuhlii in this study were highly similar to the 12S, 16S, COI and Cyt b sequences of the specimens collected from the Chinese seas in other studies.