Low-coverage MiSeq next generation sequencing reveals the mitochondrial genome of the Eastern Rock Lobster, Sagmariasus verreauxi

Abstract

The complete mitochondrial genome of the Eastern Rock lobster, Sagmariasus verreauxi, is reported for the first time. Using low-coverage, long read MiSeq next generation sequencing, we constructed and determined the mtDNA genome organization of the 15,470 bp sequence from two isolates from Eastern Tasmania, Australia and Northern New Zealand, and identified 46 polymorphic nucleotides between the two sequences. This genome sequence and its genetic polymorphisms will likely be useful in understanding the distribution and population connectivity of the Eastern Rock Lobster, and in the fisheries management of this commercially important species.

• Complete mitochondrial genome
• Eastern Rock Lobster
• MiSeq
• next generation sequencing
• Sagmariasus verreauxi

Acknowledgements

We thank Dr James Bell (Victoria University of Wellington) and Dr Bridget Green (Institute of Marine and Antarctic Studies, University of Tasmania) for providing tissue samples.

Declaration of interest

The authors have no interests to declare. SRD is supported by an Early Career Development Fellowship from La Trobe University and an Illumina MiSeq Grant. JMS and NPM are supported by a Securing, Food, Water and the Environment grant (La Trobe University).