Single nucleotide polymorphisms in the D-loop region of mitochondrial DNA are associated with epithelial ovarian cancer prognosis

Abstract

Objective: The single nucleotide polymorphisms (SNPs) in the displacement loop (D-loop) of mitochondrial DNA (mtDNA) is found in many types of cancers. A lot of reports show the SNPs of D-loop may be related to cancers prognosis. This study investigated whether the SNPs were associated with the outcome of epithelial ovarian cancer (EOC). Method: The D-loop region of mtDNA samples from blood were obtained from 89 patients with primary EOC, who received surgery. The 3 year survival curves were calculated by Kaplan–Meier method and compared by the log-rank test. Multivariate analysis was performed with Cox proportional hazards model. Result: The SNP sites of nucleotides 309 C/T, 324 C/G and 446 C/A were identified for prediction of post-operational survival. In an overall multivariate analysis, the alleles 309 and 324 were identified as independent predictors of EOC outcome. Conclusion: The results indicated that the SNPs in D-loop are associated with the clinical outcome of patients with EOC. The analysis of SNPs in D-loop region may help identify the patient genotype to predict prognosis of the EOC patients.

• Cancer
• D-loop
• mitochondria
• outcome
• ovarian

Acknowledgements

The authors thank the teachers of Hebei key lab of Laboratory Animal for their assistance in technical aspects and the Professor Jieying Zhang of Department of Pathology, the Second Hospital of Hebei Medical University, for confirming the Pathological type of EOC.

Declaration of interest

The authors declare that we have no competing interest. This work was supported by the Second Hospital of Hebei Medical University.