The mitochondrial genome of the black dwarf honey bee, Apis andreniformis (Hymenoptera: Apidae)

Abstract

We sequenced 17,329 bp of the black dwarf honey bee, Apis andreniformis (Hymenoptera: Apidae) mitochondrial genome (mitogenome) that lacked ∼200 bp of the A + T-rich region for the complete genomic sequence. The gene arrangement of the A. andreniformis mitogenome was identical to that of A. cerana. However, the genome contained five additional tRNA^Leu(CUN); four copies were located between tRNA^Met and tRNA^Gln, and one copy was between tRNA^Gln and tRNA^Ala, along with the typical sets of genes (13 protein-coding genes, 22 tRNAs, and 2 rRNAs) including regular tRNA^Leu(CUN) and the A + T-rich region (at least 923 bp). Only one copy of tRNA^Leu(CUN) differed by 1 bp from the other four copies of tRNA^Leu(CUN). Each additional tRNA^Leu(CUN) was followed by a nearly identical 68-bp long repeat sequence (95.6% identity). All 13 protein coding genes had typica start codons found in insect mitochondrial protein coding genes (two ATA, nine ATT and two ATG).

• Apis andreniformis
• mitochondrial genome
• tandem repeat sequences
• tRNA^Leu(CUN)

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this manuscript. This work was supported by a grant from the Next-Generation BioGreen 21 Program (no. PJ009031), Rural Development Administration, Republic of Korea.