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Abstract
The genomic DNA of two Provanna sp. individuals was extracted, sequenced using an Illumina HiSeq1500, and the mitogenome was assembled. The nearly complete metogenome was 16,183 bp in length, consisting of 37 typical metazoan mitochondrial genes with the typical caenogastropod mitochondrial gene order. All mitochondrial genes were encoded on the heavy strand with the exception of eight transfer RNA genes. To reconstruct the phylogenetic position of the deep-sea Provannidae, we used amino acid sequences of all the 13 mitochondrial protein-coding genes in Bayesian inference, maximum likelihood, and maximum parsimony analyses with sequences of selected gastropods. The resultant phylogenetic trees supported the placement of Provannidae in the superfamily Abyssochrysoidea of the clade Littorinimorpha of Gastropoda, providing new data for understanding the phylogeny of these deep-sea snails.
Acknowledgements
The authors thank Chinese Ocean Mineral Resources Research and Development Association (COMAR) and National Deep Sea Center of China for inviting J. -W. Qiu to participate in the cruise, the pilots and operation team of the Jiaolong’s, and the captain and crew of R/V Xiangyanghong 9 for their hospitality and support, and Dr. Hiromi Watanabe for helpful discussion.
Declaration of interest
Financial support for the sequencing and laboratory analysis was provided by Hong Kong Baptist University (FRG2/13–14/009) and the Strategic Priority Research Program of Chinese Academy of Sciences (XDB06010102). The authors alone are responsible for the content and writing of the paper.
Supplementary material available online at http://biol.hkbu.edu.hk/?page_id=823
Supplementary Figures S1–4, Tables S1–2, and Methods with References.