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Abstract
Polymerase chain reaction (PCR), long-and-accurate PCR and directly sequencing by primer walking was used to sequenced he complete mitochondrial genome sequence of Grumgzimailo's toad- headed agama, Phrynocephalus grumgrizimailoi. The Genbank accession was KM093859. There was 16,301 bp in length of the entire mitochondrial genome of P. grumgrizimailoi and the content of A, T, C, and G were 36.4%, 26.5%, 25.0% and 12.1%, respectively, that was similar to most vertebrate. The complete mitochondrial genome of P. grumgrizimailoi contain 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, plus 2 control regions and was similar to those of other Phrynocephalus sand lizards in gene arrangement and composition, except P. przewalskii and P. versicolor. The complete mitochondrial genome of P. grumgrizimailoi provided fundamental data for resolving phylogenetic relationship problems related to Agaimidae and genus Phrynocephalus.
Declaration of interest
The authors report no conflict of interest. The authors alone are responsible for the content and writing of the article. This work was supported by the National Natural Science Foundation of China (31301889), the Ph.D. Programs Foundation of Ministry of Education of China (20120211120040) and the Fundamental Research Funds for the Central Universities (lzujbky-2014-97).