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Abstract
In this study, we determined the complete mitochondrial DNA sequence of the hybrid of Megalobrama amblycephala (♀) × Megalobrama terminalis (♂) for the first time. The complete mitochondrial genome of the hybrid bream was sequenced to be 16,623 bp in size in accordance with the female parent, M. amblycephala. The genome contained 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 2 main non-coding regions (the control region and the origin of the light strand replication). Sequence alignment between the mitochondrial genomes of the hybrid and its female parent showed that a total of 23 mutation sites were found in 10 genes or regions, in particular, six sense mutations in four protein-coding genes (including COX1, ATP6, ND6 and Cytb). The genome information presented here may play an important role in further study on the genetic mechanisms of mitochondrial DNA in hybrids.
Declaration of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.
This work was financially supported by National Natural Science Foundation of China (No. 31201988 and 31472271), Modern Agriculture Industry Technology System Construction Projects of China titled as-Staple Freshwater Fishes Industry Technology System (No. CARS-46-05), Hubei Cooperation and Exchanges (No. 2013BHE006), Excellent Youth Foundation of Hubei Scientific Committee (No. 2013CFA032) and Fundamental Research Funds for the Central Universities (No. 2013PY066 and 2014PY013).