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Abstract
We have created barcode library for common Argulus spp. infecting Carassius auratus, which could also be used to identify premature forms of Argulus spp. even by non-professionals. Infected C. auratus was examined and purchased from ornamental fish-trading centers and the adult life stage of Argulus spp. was identified and DNA barcoded. The eggs of Argulus spp. were collected using bottle implants. The collected eggs are barcoded and precisely identified by matching with the adult sequences. Four species of adult Argulus spp. were identified, namely Argulus japonicus, Argulus indicus, Argulus siamensis, and Argulus foliaceus. Precise identification of egg samples was done by two different analyses, namely (i) BLAST analysis and (ii) phylogenetic clustering of adults and eggs. All egg samples including the control were precisely identified by BLAST analysis and the results are consistent with phylogenetic clustering of adult and egg’s DNA barcodes. In order to establish the DNA barcode technology for the identification of all Argulus spp and its premature forms, the development of full-fledged barcode library that includes all species of this genus is very important for the benefit of ornamental fish industries.
Acknowledgements
The authors are thankful to the head of the institute for support and encouragement and university authorities for the facility provided. Authors are thankful to the reviewer for improving the quality of the manuscript.
Declaration of interest
The authors report that they have no conflicts of interest. First author thanks University Grant Commission, Government of India, for financial support through UGC-CPEPA project.