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Abstract
In this study, the complete mitochondrial genome (mitogenome) sequence of Pholis nebulosus has been determined by long polymerase chain reaction and primer-walking methods. The mitogenome is a circular molecule of 16 524 bp in length, including the typical structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 non-coding regions (L-strand replication origin and control region), the gene contents of which are identical to those observed in most bony fishes. Within the control region, we identified the termination-associated sequence domain (TAS), and the conserved sequence block domain (CSB-F, CSB-E, CSB-D, CSB-C, CSB-B, CSB-A, CSB-1, CSB-2, CSB-3).
Acknowledgements
We are grateful to Mr. Hongyan Liu for the sample collection.
Declaration of interest
This study was supported by the China Postdoctoral Science Foundation (2013M540566) and the Public Science and Technology Research Funds Projects of Ocean (201305043). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.