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Mitochondrial DNA Part A
DNA Mapping, Sequencing, and Analysis
Volume 27, 2016 - Issue 6
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Mitogenome Announcement

The complete mitochondrial genome of the king horseshoe bat (Rhinolophus rex) using next-generation sequencing and Sanger sequencing

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Pages 4545-4546 | Received 01 Sep 2015, Accepted 09 Sep 2015, Published online: 05 Nov 2015
 

Abstract

The king horseshoe bat (Rhinolophus rex) is endemic to China. Here, we characterized the complete mitochondrial genome of R. rex using next-generation sequencing and Sanger sequencing. The mitogenome is 16 845 bp in length and contains 22 tRNAs, two rRNAs, 13 protein-coding genes, and a non-coding control region. Our traditional Sanger sequencing verified that mtDNA sequences generated from next-generation sequencing are reliable. The mitogenome of R. rex will be useful for the conservation genetics of this endangered species.

Acknowledgements

The authors thank Zhang Junpeng, Zhu Guangjian, and Meng Fanxing for assistance with field collection. The authors also thank Zhang et al. for the primers used in this work.

Declaration of interest

The authors have no interests to declare. This work was supported by a startup grant from East China Normal University for X Mao.

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