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Abstract
In this paper, the complete mitochondrial genome of Anoplophora chinensis is sequenced and reported for the first time. The mitochondrial genome is a circular molecule of 15 805 bp, 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region. Nine protein-coding genes and 14 tRNA genes are encoded on the H strand, and the other four protein-coding genes and eight tRNA genes are encoded on the L strand. The arrangement of genes is identical to all know long-horn beetles mitochondrial genomes. The nucleotide composition of the A. chinensis mitogenome is strongly biased toward A + T nucleotides (77.65%). Finally, the phylogenetic relationships of 12 Lamiinae species were reconstructed based on nucleotide sequences of COI using the Bayesian inference method. These molecular-based phylogenies support the traditional morphologically based view of relationships within the Lamiinae.
Acknowledgements
The authors thank Shuyan Wang and Naiyi Liu for helpful experiment, corrections and comments regarding this manuscript.
Declaration of interest
This project was supported by the Foundation for Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (No. 2012BAK11B03) and the Foundation for Young and Key Teachers Project (No. 02303301). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.