Next-generation sequencing yields the complete mitochondrial genome of the mummichog, Fundulus heteroclitus

Abstract

Fundulus heteroclitus (Actinopteri, Cyprinodontiformes, Fundulidae), with a remarkable tolerance to osmotic stress and water temperatures, are regarded as a significant evolution model. Herein, we report the assembled complete sequence of the mummichog mitochondrial genome based on the next-generation sequencing data. The mitogenome is determined to be 16 528 bp in length and shows an organization typical of vertebrate mitochondrial genomes, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 control region (D-loop). Overall GC content of the genome is 39.72%. Using Oryzias latipes as the outgroup, the phylogenetic analysis of 16 complete mitochondrial genomes from Cyprinodontiformes showed that F. heteroclitus together with other three Fundulus species form a cluster with strong bootstrap supports. The genus Fundulus is closely related to the genus Xenotoca.

• Killifish
• Illumina
• mitogenome
• phylogenetics

Acknowledgements

The authors would like to thank the editor and anonymous reviewers for providing valuable comments on the manuscript.

Declaration of interest

The authors have declared that no conflict of interest exists. The authors alone are responsible for the content and writing of the paper. This work was supported by Dr. Start-up fund of Liaoning Normal University (to T. Zhu).