The phylogenetic placement of Odontobutis obscura base on complete mitochondrial DNA sequence

Abstract

In our research, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of Odontobutis obscura. The aim of our research was to provide some important genetic background information for conservational practice of these valuable fish species. The total length of the mitochondrial genome is 16 864 bp and deposited in the GenBank with accession numbers KT438552. The gene arrangement and transcriptional direction were similar to other bony fishes which contained 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region. The nucleotide skewness for the coding strands of O. obscura (GC-skew = −0.26) is biased toward G and the negative GC-skew ranges from −0.51(ND2) to −0.24(CO1). The phylogenetic analysis showed that the Gobiiformes could be divided into three groups, Eleotridae, Odontobutidae and Rhyacichthyidae. The O. Obscura and O. Potamophila are sister species and clustered together.

Keywords:

• GC-skew
• mitochondrial DNA
• phylogenetic analysis
• Odontobutis obscura

Declaration of interest

The authors report no conflicts of interest and are responsible for the content and writing of the paper. This work was supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2014BAC09B00) and International S&T Cooperation Projects of China (2015DFA90450).