Microsphere-embedded cryogel for selective and efficient depletion of immunoglobulin G from human serum

Abstract

In this study a novel composite cryogel column was synthesized for affinity depletion of immunoglobulin G (IgG) from human serum. The microsphere-embedding technique combines the large surface area of microspheres with excellent properties of cryogels. The poly(hydroxyethyl methacrylate- N-methacryloyl-L-histidine methyl ester) microsphere (2 μm size)- embedded composite cryogel (MECC) column was synthesized and characterized by various methods. The specific surface area of the composite cryogel was found as 92 m²/g by using BET measurement. Maximum HIgG adsorption on MECC column was found as 50.5 mg/g. MECC column can be reused many times with no apparent decrease in HIgG adsorption capacity.

Keywords::

• Composite
• cryogel
• IgG affinity depletion
• microsphere embedding
• protein adsorption

Acknowledgments

The authors wish to thank the National Nanotechnology Research Center at Bilkent University for providing the SEM images.

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.