A novel amperometric biosensor based on ZnO nanoparticles-modified carbon paste electrode for determination of glucose in human serum

Abstract

Zinc oxide nanoparticles-(ZnONPs)modified carbon paste enzyme electrodes (ZnONPsMCPE) were developed for determination of glucose. The determination of glucose was carried out by oxidation of H₂O₂ at +0.4 V. ZnONPsMCPE provided biocompatible microenvironment for GOx and necessary pathway of electron transfer between GOx and electrode. The response of GOx/ZnONPsMCPE was proportional to glucose concentration and detection limit was 9.1 × 10^–3 mM. K_m and I_max, were calculated as 0.124 mM and 2.033 μA. The developed biosensor exhibits high analytical performance with wide linear range (9.1 × 10^–3–14.5 mM), selectivity and reproducibility. Serum glucose results allow us to ascertain practical utility of GOx/ZnONPsMCPE biosensor.

Keywords::

• amperometric biosensor
• carbon paste electrode
• glucose
• human serum
• ZnO nanoparticles

Acknowledgement

Financial support of this study from The Scientific and Technological Research Council of Turkey is gratefully acknowledged.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.