Abstract
Methemoglobin concentration is an important pathophysio-logical biomarker, reflecting the oxygen-carrying and oxygen-releasing capabilities of hemoglobin (Hb). Raman spectroscopy is used to develop a novel technique for determining the methemoglobin concentration. Raman activity combined with two-dimensional correlation analysis is an attractive method for investigating Hb oxidation, exhibiting several relevant peaks in the range of 1200–1650 cm− 1. Methemoglobin concentration is estimated by measuring the intensity of Raman peaks in the ranges of 1210–1230 cm− 1 and 1340–1380 cm− 1 with 785-nm excitation. The correlation between Raman-based methemoglobin concentration estimations and the methemoglobin concentration measured using spectrophotometry was highly significant. These results suggest the potential of Raman spectroscopy as a new quantitative approach to determine the methemoglobin concentration.
Declaration of interest
The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.
This work was supported by grants from the National Natural Science Foundation of China (No. 31271001), the National key scientific research projects of China (No. 2012CB933001) and the National High Technology Research and Development Program of China (No. 2012AA021902).