Preparation and optimization of quercetin-loaded liposomes for wound healing, using response surface methodology

Abstract

The basic objective of this study was to prepare quercetin-loaded liposomes by the thin film hydration method. The liposomal formulation was optimized using response surface methodology (RSM). A rotation speed of 75 rpm and a water bath temperature of 46°C were finalized as the best for optimized drug-loaded liposomal formulation. In vitro characterization of the quercetin-loaded liposomal formulation was done through some parameters including the entrapment efficiency (EE), drug release (DR), and mean particle size; the resulting values of 86.5 ± 0.42%, 76.5%, and146 nm were found to be standard characterized values respectively. It is concluded that quercetin-loaded liposomal formulations achieve sustained release of drug in wound areas.

Keywords::

• liposome
• quercetin
• response surface methodology
• temperature

Acknowledgments

The authors are thankful to the Director, University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur, Chhattisgarh, India, for providing necessary infrastructural facilities.

Declaration of interest

The authors declare that there are no conflicts of interest regarding the publication of this paper.

The authors would like to thank and acknowledge UGC-MRP-41-748-2012, UGC-RA-70371/2012, DST-FIST and UGC-SAP for providing financial support for this work.