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Abstract
Objective: To study the cellular compatibility of the magnetic nanocomposite (n-HA/PLLA/Fe2O3) and to discuss the biological behaviors of cells including surface adhesion, proliferation, and expression. The present work provides an experimental basis for medical application. Method: Rat osteoblasts (OB) were co-cultured with the magnetic nanocomposite. Cell proliferation, cell adhesion, and the expression of type I collagen (Col-I) and osteocalcin (OCN) gene were characterized by the cell counting kit-8 (CCK-8) method, scanning electronic microscopy (SEM), and reverse transcription polymerase chain reaction (RT-PCR), respectively. Results: CCK-8 detection showed that there was no difference in cell proliferation on the magnetic nanocomposite between the experimental group and control group (P > 0.05). SEM indicated that a large amount of cells adhered to the surface and in the pores of the magnetic nanocomposite. As the co-culture time increased, the cells adhering to the magnetic nanocomposite showed an obvious increase. RT-PCR detection showed that as the co-culture time increased, the expression of the Col-I gene was enhanced (P 0.05). There was no obvious difference in the expression of the OCN gene (P > 0.05). Conclusion: The magnetic nanocomposite is suitable for cell adhesion, growth, and differentiation with a high cellular compatibility.
Acknowledgment
This study received financial support from Guangdong Science and Technology Project (the project number is 2013B021800100), and the Shenzhen Science and Technology Project (the project numbers are CXZZ20130321152713220 and JCYJ20140414170821164).
Declaration of interest
The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.