Polycaprolactone-based in situ implant containing curcumin-PLGA nanoparticles prepared using the multivariate technique

Abstract

Studies on the effect of curcumin/PLGA ratio (CPR), stabilizer (PVA) concentration, homogenization speed, homogenization time, and sonication time on mean particle size (MPS) and percentage drug encapsulation (PDE) were performed using the multivariate technique. MPS and PDE were found to be more dependent on the interaction of sonication time with the other variables. Curcumin was released in a sustained manner from curcumin-PLGA nanoparticles (CPN). CPN improved the survival rate of Ehrlich ascites carcinoma (EAC)-bearing mice and controlled the EAC-induced change in hematological parameters. Histopathology of vital organs showed that the formulation was safe. Polycaprolactone was used in preparing an in situ implant containing CPN.

Keywords::

• Box–Behnken design
• design of experiment
• Ehrlich ascites carcinoma
• experimental design
• factorial design
• quality by design

Acknowledgement

The authors would like to acknowledge the authorities of Manipal University for providing the necessary facilities required for carrying out this experiment.

Conflict of interest

All the authors included in the manuscript declare that there is no conflict of interest regarding the material discussed in the manuscript with any of the financial organizations.